ABSTRACT
<p><b>OBJECTIVE</b>To reveal the mechanism of sterility of Liriope spicata var. prolifera.</p><p><b>METHOD</b>Meiotic behavior and pollen development of L. spicata var. prolifera were described in detail. Its proto-variety L. spicata was also investigated for comparison.</p><p><b>RESULT</b>During the meiosis of microspore mother cells (MMC), most of cells displayed normally in L. spicata, but abnormally in L. spicata var. prolifera. The abnormity was showed that: some chromosomes or their fragments moved out of the spindle of the cell at metaphase; some chromosome bridges, fragments and lagging ones were formed at anaphase; and many microspores displayed the micronucleus at the telophase. The pollen development was abnormal in L. spicata var. prolifera and normal in L. spicata, with the aberration rate of pollen was 95.81% and 3.44% separately.</p><p><b>CONCLUSION</b>These results indicate that some abnormalities of meiotic behavior and pollen development are main reasons for inducing microspore abortion during its development.</p>
Subject(s)
Liriope Plant , Cell Biology , Physiology , Meiosis , Physiology , Plants, Medicinal , Cell Biology , Physiology , Pollen , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To study the technique of the callus induction from anther and plant regeneration of medicinal plants Liriope spicata var. prolifera.</p><p><b>METHOD</b>Callus was induced from anther of L. spicata var. prolifera on a MS medium supplemented with different hormones. The squash methods combined with a microscope were used to analyze chromosomes of regenerated plantlets.</p><p><b>RESULT</b>MS +2,4-D 1.0 mg x L(-1) + KT 2.0 mg x L(-1) gave the highest induction ratio which was 41.07%. MS +6-BA 1.5-2.0 mg x L(-1) + NAA 0.1-0.3 mg x L(-1) was suitable for the induction and proliferation of indefinite buds. The buds were transferred to 1/2 MS medium supplemented with NAA 0.1-0.3 mg x L(-1) for rooting. The shoots produced roots of culture and formed complete plantlets. The regenerated plantlets originated from somatic cells. At the same time, the effects of pretreatment of low temperature at 4 degrees C on the callus induction were studied and discussed.</p><p><b>CONCLUSION</b>This paper sets up the method of tissue culture of anther somatic-cells and intermediate propagation of L. spicata var. prolifera.</p>